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- #Make The Cut 4.1.0 Serial serial
- #Make The Cut 4.1.0 Serial professional
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Turn the Petri dish through 90 o anticlockwise, then streak the wire loop across the surface of the agar into the centre of the plate. Turn the Petri dish through 90 o anticlockwise again and streak across the surface of the agar in three parallel lines.Ĥ. Use the cooled wire loop to streak the agar plate across the surface in three parallel lines.Ī small amount of culture must be carried over.ģ. Turn the Petri dish through 90 o anticlockwise. Remove the wire loop and close the Petri dish.įlame the wire loop and allow it to cool.Ģ. Smear the inoculum backwards and forwards across a small area of the agar medium on the left hand side of the plate.
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Hold the charged wire loop parallel with the surface of the agar. Then they lift out the base, invert it, then inoculate the agar facing up.ġ.Partially lift the lid of the Petri dish containing the solid medium.
#Make The Cut 4.1.0 Serial professional
Professional microbiologists start with the Petri dish inverted on the desk. Streaking causes a progressive dilution of an inoculum over the surface of solidified agar medium in a Petri dish, so that the colonies of bacteria or yeast grow separated from each other as single isolated They must be made of non-absorbent cotton wool, be kept dry, and must keep its shape after being removed and returned to the test-tube. Label test-tubes and bottles with a marker pen where it will not rub off.Ĭotton wool plugs are used to plug test-tubes and pipettes to allow the passage of air, but prevent the passage of micro-organisms. Remove the cap of the bottle or cotton wool plug with the little finger of the right hand.ĭo not put the cap or cotton wool plug down on the desk.įlame the neck of the bottle or test-tube by passing the neck forwards and back through a hot Bunsen burner flame.Īfter the procedure, replace the cap on the bottle or cotton wool plug using the little finger. Lift the bottle or test-tube with the left hand. Never use the mouth to "suck up" fluid into a pipette!įlame the neck of bottles and test-tubes. Immediately after use, put the "contaminated pipette" into a discard pot of 0.25% v/v sodium chlorate I (sodium hypochlorite), then remove the teat. Keep the pipette tip beneath the liquid surface while taking up liquid, to avoid taking up air bubbles. Return any excess fluid if a measured volume is required.
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Leave free the finger to take hold of the cotton wool plug or cap of a test-tube or bottle.ĭepress the teat carefully to take up enough fluid, but not enough to wet the cotton wool plug. Hold the pipette barrel as you would a pen, but do not grasp the teat. Remove the pipette from its container or wrapper by the end that contains a cotton wool plug.
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Use a sterile graduated pipette and filler or dropping (Pasteur) pipette to transfer cultures, sterile media and sterile solutions. Sterilize the wire loop again immediately after use. Move the rest of the wire slowly upwards into the hottest region of the flame, above the light blue cone, and hold it there until it is red hot.ĭo not put the wire loop down on the desk and do not wave it around in the air. Hold the handle end of the wire in the light blue cone of the flame, the cool area of the flame. Heat the end of the loop slowly, because after use it may hold culture that may splutter on rapid heating. Hold the handle of the wire loop close to the top, like holding a pen, at an almost vertical angle, leaving the little finger free to take hold of the cotton wool plug or screw cap of a test-tube or bottle. Sterilize wire loops by heating in a Bunsen burner flame until red.
#Make The Cut 4.1.0 Serial serial
School Science LessonsĤ.1.1 Prepare colonies of different micro-organismsĤ.1.2 Enrichment of wild yeast strains, Aspergillus nigerĤ.3.1 Grow African violet, with in vitro cultureĤ.3.2 Grow African violet, from pieces of leafĤ.3.3 Grow Gerbera using in vitro cultureĤ.1.2.5 Prepare heat-fixed stained bacterial smearsĤ.1.7 Prepare streptomycin using Streptomyces griseusĤ.4.9 Presence of bactericidal substances using a coin and Bacillus mycoidesĤ.1.2.3 Aseptic transfer of bacterial cultures from a bottle or tubeĤ.1.2.4 Aseptic transfer of bacterial cultures from a culture plateĤ.1.10 Colony counts using the calibrated drop methodĤ.1.8 Microbial decomposition of cigarette paperĤ.1.2.5 Prepare heat-fixed stained bacterial smears.Ĥ.1.2.10 Serial decimal dilution of a bacterial suspensionĤ.1.2.7 Streak dilution plate method for pure cultures from a mixed suspensionĤ.9.2.3 Tensides, alkylphenol ethoxylatesĤ.2.10 Measure the amount of juice from apple mash with and without pectinase.Ĥ.2.11 Prepare lactose from milk or whey using immobilized lactaseĤ.2.6 Prepare vinegar with Acetobacter acetiĤ.2.4 Prepare wine from grape juice and vinegar from wineĤ.3.17 Prepare yoghurt, test milk quality Microbiology, Fermentation, Food preparation.